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Two photon microscopy of in vivo brain function

Two photon microscopy of in vivo brain function
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Two-photon microscopy of in vivo brain function. (a) Basic mechanism of two-photon fluorescence. (b) Schematic of surgical preparation of exposed cortex, with sealed glass window and microscope objective positioning. Green dot shows location of two-photon fluorescence. (c) Examples of two-photon maps of the vasculature following intravenous injection of dextran-conjugated fluorescein. Black dots and stripes show red blood cell motion. (d) Dual-channel imaging of neuronal (green) and vascular (red) signals: (left) Oregon Green 488 BAPTA-1 AM calcium sensitive dye stained neurons and (right) transgenic mouse expressing green fluorescent protein (GFP) in a subpopulation of neurons (mouse supplied by Jeffrey M. Friedman, Rockefeller University, New York) 101. Texas dextran red is the intravascular tracer in both cases. (e) Three channel imaging of Tg2576 APP Alzheimer's disease mouse model with amyloid-targeting dye (blue), GFP expressing neurons and dendrites (green) and vasculature (red). Adapted from 52 and contributed by Elizabeth Hillman (Columbia University, New York). Kherlopian et al. BMC Systems Biology 2008 2:74 doi:10.1186/1752-0509-2-74

Source: Armen R Kherlopian1 , Ting Song2 , Qi Duan2 , Mathew A Neimark2 , Ming J Po2 , John K Gohagan3 and Andrew F Laine2,4 via wikipedia

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 - Hochgeladen am 17.02.2011
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